Composite

Part:BBa_K648019:Design

Designed by: Jim Rose   Group: iGEM11_Penn_State   (2011-07-05)


Fast-Fusion GFP-LacZa Reporter (TEV cleavage with long linker)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 704
    Illegal BsaI.rc site found at 871


Design Notes

All parts were assembled via standard 25 assembly methods


Source

The design of this part was inspired by the Imperial College London 2010 iGEM Team's Fast-Response module, part BBa_K316007.

References